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KMID : 0545120190290122006
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Journal of Microbiology and Biotechnology
2019 Volume.29 No. 12 p.2006 ~ p.2013
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Improving Pneumovirus Isolation Using a Centrifugation and AZD1480 Combined Method
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Lee Han-Saem
Woo Hye-Min
Kim Ki-Soon
Park Se-Hee
Park Man-Seong
Kim Sung-Soon
Kim You-Jin
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Abstract
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The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.
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KEYWORD
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Virus isolation, centrifugation, AZD1480, human respiratory syncytial virus, human metapneumovirus
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